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Image Search Results
Journal: Stem Cells and Development
Article Title: Microgravity Reduces the Differentiation and Regenerative Potential of Embryonic Stem Cells
doi: 10.1089/scd.2015.0218
Figure Lengend Snippet: Embryoid body (EB) formation and recovery post-μg exposure. mESCs ( A ) were used to form EBs ( B ) for analysis of early lineage commitment and differentiation during and after 15 days spaceflight. EBs from 1xg bioreactor controls ( C, E ) and those recovered post-μg exposure ( D, F ) showed similar appearance both in large sheets adherent to bioreactor fibers ( C, D ) and in smaller more EB-like cell clusters ( E, F ). No significant differences were found in the number of EBs that were recovered and adhered to collagen, COL, fibronectin, FN ( I ) and tissue culture-treated plastic, TC ( J ) matrices following differentiation in μg ( H ) compared to 1xg; ( G ) conditions. Scale bar = 100 μm ( A ) or 500 μm ( B–H ). 1xg, n = 5; μg n = 8. 1xg, Earth's gravity; mESC, mouse embryonic stem cell; μg, microgravity; TC, tissue cultures.
Article Snippet: Cells were cultured within
Techniques: